Genetic and environmental perturbations of human cell lines as an in vitro model for gene-environment interaction
Mi Huang finished his bachelor education in pharmacy (2014) and master education in pharmaceutical chemistry (2017) in South-central University for Nationalities in China. Before he joint Professor Paul Frank´s team, he had been focusing on the discovery of natural medicines against type 2 diabetes from traditional Chinese herbs. In 2018, he started his PhD study in GAME unit under the supervision of professor Paul Franks. His PhD project will take two defined approaches to identifying gene targets for experimentation. The first will be to use the strongest of our previously published candidates for gene x environment associations. The second approach will apply a hypothesis-free method by using several functional genomics techniques, such as ATAC-Seq, ChIP-Seq and 3D chromosomal capture, to identify lifestyle mimetic-responsive hotspots in the genome that may harbor functional mutations. After identification of a specific target for testing, CRISPR will be applied in the appropriate cell type. As this work will be focusing on type 2 diabetes, tissues of relevance include skeletal muscle, adipose, liver and beta-cells. Experience of working with these cell types is established at our institute.
Research project participant